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DOI: 10.5593/SGEM2016/HB63/S08.049

THE INFLUENCE OF BACTERICIDAL VIRUCIDAL CONCENTRATIONS OF THE DISINFECTANTS ON TECHNOLOGICAL PARAMETERS OF BOVINE BLOOD PLASMA CONTAINING ANTIBODIES TO THE VIRAL AGENTS

A. K. Barsukov, A. I. Kuznetsov, K. K. Sharafullin
Friday 11 November 2016

References: 16th International Multidisciplinary Scientific GeoConference SGEM 2016, SGEM Vienna GREEN Extended Scientific Sessions, www.sgemviennagreen.org, SGEM2016 Conference Proceedings, ISBN 978-619-7105-79-7 / ISSN 1314-2704, 2 - 5 November, 2016, Book 6 Vol. 3, 379-386pp, DOI: 10.5593/SGEM2016/HB63/S08.049

ABSTRACT
For examining the possibility of storing up blood and its separation, slaughtering blood plasma was gathered in the slaughterhouse. Such disinfectant as phenol or quaternary ammonium salts (compounds) were added in its composition up to the final concentrations provided by the instructive and methodological or technical research recommendations. During 20 days of observation of the level of bacterial contamination, the amount of premanufactured protein spectrum including the distribution between sediment and supernatant fractions of the infectious bovine rhinotracheitis virus (the IBRV) genome and the activity of antibodies to the IBRV and to the leukemia virus (the LV) in cattle was determined. We also studied the influence of used disinfectants on trypsin activity in order to identify their potential inhibitory effect on hydrolysis of plasma proteins under endogenous protease condition. It was found that high concentrations of the disinfectants having virucidal and bactericidal properties cause protein aggregation in the earliest periods of the observation until the precipitation or gelling of the plasma samples. Maximum permissible concentration of phenol in the plasma composition reduces trypsin activity in the model system for (25 ± 5)%. Any similar inhibitory effect on trypsin activity for the maximum permissible concentration of quaternary ammonium salts (compounds) has not been found. The PCR analysis of the sediment and the supernatant induced by the disinfectants has not revealed the selective co-precipitation genomes of the IBRV. Also the influence of maximum permissible concentrations of the disinfectants on the activity of IgG class antibodies specific to the IBRV and the LV in cattle has not been found. We believe it is possible to use optimal concentrations of the disinfectants in the composition of fractionating solutions designed to form reactor establishment at the place of storing up the premanufactured samples and its further separation under non-sterile conditions.

Keywords: disinfectants, aggregation of plasma proteins, protease activity, virus-specific antibodies.

PAPER DOI: 10.5593/SGEM2016/HB63/S08.049, THE INFLUENCE OF BACTERICIDAL VIRUCIDAL CONCENTRATIONS OF THE DISINFECTANTS ON TECHNOLOGICAL PARAMETERS OF BOVINE BLOOD PLASMA CONTAINING ANTIBODIES TO THE VIRAL AGENTS

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