DBPapers
DOI: 10.5593/SGEM2016/B61/S25.097

RESEARCHES OF SPERM CRYOPRESERVATION ON POLIODON (POLYODON SPATHULA) FOR OPTIMIZATION OF SPAWNING BIOTECHNOLOGY

M.Costache
Wednesday 7 September 2016 by Libadmin2016

References: 16th International Multidisciplinary Scientific GeoConference SGEM 2016, www.sgem.org, SGEM2016 Conference Proceedings, ISBN 978-619-7105-68-1 / ISSN 1314-2704, June 28 - July 6, 2016, Book6 Vol. 1, 737-742 pp

ABSTRACT

The paper presents the experiments of sperm cryopreservation on poliodon (Polyodon spathula), recently acclimated sturgeon in Romania. Hormonal induction was accomplished using Nerestin 5A as follow: males were injected intramuscular with Nerestin 5A at a dose of 0.1 ml/kg body weight.Females were injected with Nerestin 5A in a total dose of 1.2 ml/kg administered in a preliminary injection (0.2 ml/kg) and a releaser injection (1.0 ml/kg) within 12 hours. The eggs were harvest by MIST method [5]. The accomplished research has been to determine the effect of using methanol (MeOH) and dimethyl sulfoxide (DMSO) as cryoprotectants in combination with two extenders (modified Tsvetkova’s extender: mT and modified Hanks’ balanced salt solution: mHBSS) on the spermatozoa motility and fertilization and hatching percentage of eggs by use of thawed sperm.The best post thawed motility (78 ± 5 %) was recorded using modified Tsvetkova’s extender: mT with 10 % MeOH, and the best fertilization and hatching percentage of eggs were achieved by using mT extender in combination with 5 % MeOH. The results confirm data from literature (Detlaf et al. 993, Mims et al. 2000, Akos Horvath et al. 2006) reporting that MeOH is a proper cryoprotectant for freezing poliodon sperm

Keywords: cryopreservation, fertilization, motility, poliodon, spawn