DBPapers
DOI: 10.5593/SGEM2016/B61/S25.087

NITROGEN AND PHOSPHORUS REMOVAL FROM WASTEWATER TREATMENT PLANT OUTLET USING IMMOBILIZED CHLORELLA SOROKINIANA UTEX 1230

R.G. Manea, M.C. Iordan, I. I. Ardelean
Wednesday 7 September 2016 by Libadmin2016

References: 16th International Multidisciplinary Scientific GeoConference SGEM 2016, www.sgem.org, SGEM2016 Conference Proceedings, ISBN 978-619-7105-68-1 / ISSN 1314-2704, June 28 - July 6, 2016, Book6 Vol. 1, 661-668 pp

ABSTRACT

It is well known that nitrogen and phosphorus removal using classical protocol using activated sludge is not very efficient; one alternative is the use of chemical flocculants to encompass rest of the phosphorus. In the last decade there is a growing interest in the use of photosynthetic microorganisms to consume nitrogen and phosphorus containing chemicals from the wastewater treatment plant outlet. The purpose of this research is to use immobilized biomass of Chlorella sorokiniana for the removal of total nitrogen and total phosphorus from the wastewater already treated by a wastewater treatment plant using activated sludge). Chlorella sorokiniana was immobilized in BG11- agar medium. In these experiments, different volumes (2 liters, 1 liter and 0.7 liters) of effluent water from the wastewater treatment plant were left in contact with immobilized Chlorella sorokiniana algal biomass corresponding to 141.81 mg total chlorophyll, thus changing the ratio water volume/ algal biomass, the retention time (5 days), temperature of 24 Celsius degrees and light conditions being constant all over the experiment. The results show that after 3 days immobilized Chlorella sorokiniana algal biomass remove 78% of nitrate and 88% of phosphorus, and after 5 days the removal goes up to 91% in case of nitrate and 98% for phosphorus, for the experiment using 0.7 liters of outlet water. The results expressed as mg analyte/100mg total Chl/day are higher during the first day (39.5 mg nitrate, 0.353 mg total phosphate, 11.7 mg total nitrogen, 1.59 mg ammonium and 7.05 mg O2 COD) than in the second day, with the exception of COD which is slighter higher during the second day (9.03 mg O2).

Keywords: Chlorella sorokiniana, nitrogen, phosphorus, removal, outlet wastewater