DBPapers
DOI: 10.5593/SGEM2016/B32/S13.017

FINDINGS OF RHIZOSPHERE COLONIZATION CAPACITIES BY NITROGEN FIXING BACTERIA FROM AZOTOBACTER AND CLOSTRIDIUM

A.B. Borozan, D.M. Bordea, D. Dogaru, S. Popescu, M. Horablaga
Wednesday 7 September 2016 by Libadmin2016

References: 16th International Multidisciplinary Scientific GeoConference SGEM 2016, www.sgem.org, SGEM2016 Conference Proceedings, ISBN 978-619-7105-62-9 / ISSN 1314-2704, June 28 - July 6, 2016, Book3 Vol. 2, 127-132 pp

ABSTRACT
Bacteria from rhizosphere have an important role in nutrients disponibility from soil. From chemical elements which take part in plants nutrition, nitrogen is an important agent which could limit plants growing. There are rhizosphere bacteria which are involved in nitrogen transforming in organic forms.
Association between Azotobacter and Clostridium bacteria and plants presents lately a real interest. Free nitrogen fixing bacteria from Azotobacter and Clostridium were isolated from two kinds of soils: eutricambosoil weak gleyed and eutricambosoil moderate gleyed, both of them cultivated with vetch (Vicia sativa L). Soils are from western Romania. Soil samples are taken in autumn, from 0-20 cm, from three zones: rizoplan, rhizosphere and edaphosphere. Soil samples preparation and the isolation of the two kinds of bacteria was done on a mineral medium, in lab conditions.
After one week incubation of free nitrogen fixing bacteria was observed that in moderate gleyed eutricambosoil Azotobacter was the dominant type in rhizosphere. On the other hand, in weak gleyed eutricambosoil, Azotobacter bacteria are dominant in rizoplan and edaphosphere.

Keywords: edaphosphere, Vicia sativa L, weak gleyed eutricambosoil, moderate gleyed eutricambosoil

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