DBPapers
DOI: 10.5593/SGEM2014/B61/S25.061

NATURAL INTERSPECIFIC ESCHERICHIA-PROTEUS GENETIC TRANSFORMATION IN WASTE WATER CONDITIONS VERSUS URINE

Lepadatu A.C., Negreanu-Pоrjol T. Sanda J., Miresan H., Negreanu-Pоrjol B.S.
Wednesday 1 October 2014 by Libadmin2014

References: 14th International Multidisciplinary Scientific GeoConference SGEM 2014, www.sgem.org, SGEM2014 Conference Proceedings, ISBN 978-619-7105-20-9 / ISSN 1314-2704, June 19-25, 2014, Book 6, Vol. 1, 443-450 pp

ABSTRACT
Based on cultural and biochemical characteristics, a number of uropathogen strains of Escherichia coli and Proteus sp. have been isolated and identified. For this isolated strains, the spectrum of sensitivity - resistance against 10 antibiotics has been determined. One pathogenic strain of E. coli has been randomly chosen, sensitive to all tested antibiotics. This strain has been used as genetic material acceptor strain. Another uropathogen strains of E. coli and Proteus sp., both ciprofloxacin-resistant with chromosomal resistance, have been used as donor strains. Chromosomal DNA donor strains were initially subjected to a plasmidial curing process and then used in genetic transformation experiments by growing strains in common environments acceptor fluid - urine and domestic waste water - ensuring physiological conditions of temperature and calcium concentration. The frequency and the efficiency of natural intergeneric Escherichia - Proteus genetic transformation and natural interspecific Escherichia – Escherichia genetic transformation were assessed.

Keywords: natural genetic transformation, antibiotic resistance markers, uropathogen, waste water, urine, transformation frequency