DBPapers
DOI: 10.5593/sgem2017/31/S15.112

NEW POTENTIALLY TOXIC PSEUDO-NITZSHIA SPECIES (BACILLARIOPHYCEAE) IDENTIFIED BY MOLECULAR APPROACH IN THE BLACK SEA (VARNA BAY)

N. Dzhembekova, I. Atanasov, P. Ivanova, S. Moncheva
Monday 11 September 2017 by Libadmin2017

References: 17th International Multidisciplinary Scientific GeoConference SGEM 2017, www.sgem.org, SGEM2017 Conference Proceedings, ISBN 978-619-7408-04-1 / ISSN 1314-2704, 29 June - 5 July, 2017, Vol. 17, Issue 31, 889-896 pp, DOI: 10.5593/sgem2017/31/S15.112

ABSTRACT

The potentially toxic genus Pseudo-nitzschia H.Peragallo, 1900 is common for the phytoplankton community in the Bulgarian part of the Black Sea often proliferating to bloom densities. The highly similar morphological features hamper the precise species discrimination by light microscopy and an accurate identification requires the application of more sophisticated methods. In this study we used genus specific large subunits ribosomal DNA (LSU rDNA) primer pair in order to elucidate the species diversity within Pseudo-nitzschia in Varna Bay. Three Pseudo-nitzschia species (four distinct ribotypes) were found in the constructed from environmental samples clone libraries. Molecular data were compared with light microscopy counts. This is the first detection of P. linea Lundholm, Hasle and G.A.Fryxell, 2002 in the Black Sea and first confirmation of presence of P. calliantha Lundholm, Moestrup & Hasle, 2003 and P. pungens var. aveirensis Lundholm, Churro, Carreira & Calado, 2009 in Bulgarian waters. The results from this preliminary study are in support of the potential of molecular analysis as a powerful approach for clarifying Pseudo-nitzschia taxonomic diversity in Bulgarian Black Sea waters.

Keywords: Bulgarian Black Sea coast, clone libraries, environmental DNA, genus-specific LSU rDNA primers, Pseudo-nitzschia