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LYSOSOMAL DESTABILISATION IN THE MOLLUSCA BIVALVIA AS INDICATOR OF ENVIRONMENTAL DEGRADATION OF ALBANIAN SOUTHERN ADRIATIK AREA

AUTHOR/S: M. LIKA, H. MANKOLLI
Sunday 1 August 2010 by Libadmin2009

9th International Multidisciplinary Scientific GeoConference - SGEM2009, www.sgem.org, SGEM2009 Conference Proceedings/ ISBN 10: 954-91818-1-2, June 14-19, 2009, Vol. 2, 251-256 pp

ABSTRACT

The aim of the present study is to investigate the biological effects of contaminants is a
combination of chemical and biological measurements. Among biological
measurements, investigation of alteration at the cellular or sub-cellular level can provide
an early-warning signal of adverse biological response before they are manifested at the
population and community level. Lysosome destabilization test has been the method of
choice in the present paper. A relatively low retention time of the used red neutral dye
amounting at 59 min was detected at the distance of 50 m offshore indicating a high
level of the biological stress. Whereas at 200-m distance the retention time sensibly
increases at 120min. Comparably higher levels of Hg and Cr were detected at 50-m
offshore distance.

The present paper introduces an investigation of the environmental status of two marine
urban sites in the Albanian Southern Adriatic Area, using the neutral red assay for the
determination of the stability of the lysosomal membranes in the blue mussel Mytilus
galloprovincialis, Lamark, 1819.

The NRR times recorded in the granular haemocytes of Narta and Orikum mussels
showed that the seasonal variation of this biomarker did not follow a clear trend. The
labilization time of the haemocyte lysosomal membrane in the Narta mussels was
significantly (p<0.05) less during July (48.37±30.04 min) than in September
60.78±23.47 min) and January (62.13±24.33 min), whereas no significant differences
(p<0.05) were noted at this site, or even between the NRRT of the three sampling
periods of the Orikum mussels.

Keywords: Lysosome, test, biological measurements, marine, environmental status

PAPER 2009/s14.3: LYSOSOMAL DESTABILISATION IN THE MOLLUSCA BIVALVIA AS INDICATOR OF ENVIRONMENTAL DEGRADATION OF ALBANIAN SOUTHERN ADRIATIK AREA

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