ANTIOXIDANT ACTIVITY DETERMINATION OF HORTICULTURAL CROPS

Hegedus, O.; Hegedusova, A.; Szarka, K.; Slosar, M.; Matova, A.
Abstract:
This work is focused on antioxidant activity of various types of horticultural crops. Several types of vegetable and fruits were analyzed by free radicals scavenging method using 1,1-diphenyl-2-picrylhydrazyl (DPPH). In this work various methods of analysis results expressing were evaluated. The results were expressed, in terms of the widest applicability, while the simplicity of the serial analysis were maintained. Percentage of inhibition of free radicals was expressed per unit sample weight and standard pipetting volume of the extract. This preserved the simplicity and speed of the used method. At the same time, the wider applicability of the method for the most diverse horticultural crops with different coloring and antioxidant activity was achieved. The interference of the extract colorants was eliminated by reaction mixture preparing, directly in the cuvette of the spectrophotometer. The method was statistically evaluated by important validation parameters calculating. The repeatability and the relative combined standard uncertainty were calculated. The results showed that, the repeatability of the method is 8.4% and the relative combined standard uncertainty is 11.0 %.
SGEM Research areas:
Year:
2019
Type of Publication:
In Proceedings
Keywords:
antioxidant activity determination; DPPH; vegetable; fruits
Volume:
19
SGEM Book title:
19th International Multidisciplinary Scientific GeoConference SGEM 2019
Book number:
6.1
SGEM Series:
International Multidisciplinary Scientific GeoConference-SGEM
Pages:
543-550
Publisher address:
51 Alexander Malinov blvd, Sofia, 1712, Bulgaria
SGEM supporters:
Bulgarian Acad Sci; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci & Arts; Slovak Acad Sci; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; World Acad Sci; European Acad Sci, Arts & Letters; Ac
Period:
30 June - 6 July, 2019
ISBN:
978-619-7408-88-1
ISSN:
1314-2704
Conference:
19th International Multidisciplinary Scientific GeoConference SGEM 2019, 30 June - 6 July, 2019
DOI:
10.5593/sgem2019/6.1/S25.070
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