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OPTIMIZATION OF ?-1,4—GLUCOSIDASE AND ACID PHOSPHATASE ENZYME METHODS FOR AGRICULTURAL SOIL STUDIES

Galieva, G.; Selivanovskaya, S.; Galitskaya, P.
Abstract:
In our study, we optimized two methods of enzyme activity estimations characterizing C and P cycles (?-1,4-glucosidase and acid phosphatase) for agricultural soil with 2.629% of total C and 0.062 % of total N sampled in Tatarstan (Russia). Concentration of the substrates (4-MUF-?-d-glucopyranoside, 4-MUF-phosphate) in the range from 20 to 900 ?mol g-1 soil was used, four levels of buffer pH (3, 6, 8, 11) were compared, and three different dilutions of soil suspension (1%, 2%, 10% w/v) were investigated. Besides, the optimal time of measurement was chosen. For ?-1,4-glucosidase, it was revealed that 4-MUB-?-d-glucopyranoside concentration should be 400 ?mol g-1 of soil, buffer pH should be 8.0, and soil suspension should be prepared using 1 part of soil and 100 parts of water. The measurements should be done after 60 min of incubation. For acid phosphatase, it was revealed that 4-MUB-phosphate concentration should be 400 ?mol g-1 of soil, buffer pH should be 11.0, and soil suspension should be prepared using 1 part of soil and 50 parts of water. The measurements should be done after 30 min of incubation.
SGEM Research areas:
Year:
2018
Type of Publication:
In Proceedings
Keywords:
soil microbial enzyme activities; synthetic fluorescent dye-conjugated substrates; ?-1;4-glucosidase; acid phosphatase; buffer p?; soil suspension; incubation time; saturation curve.
Volume:
18
SGEM Book title:
18th International Multidisciplinary Scientific GeoConference SGEM2018
Book number:
6.4
SGEM Series:
International Multidisciplinary Scientific GeoConference-SGEM
Pages:
235-242
Publisher address:
51 Alexander Malinov blvd, Sofia, 1712, Bulgaria
SGEM supporters:
Bulgarian Acad Sci; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci & Arts; Slovak Acad Sci; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; World Acad Sci; European Acad Sci, Arts & Letters; Ac
Period:
3 – 6 December, 2018
ISBN:
978-619-7408-71-3
ISSN:
1314-2704
Conference:
18th International Multidisciplinary Scientific GeoConference SGEM2018, 3 – 6 December, 2018
DOI:
10.5593/sgem2018V/6.4/S08.030
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